Injectable hyaluronic acid and platelet lysate-derived granular hydrogels for biomedical applications

Towards the repair of damaged tissues, numerous scaffolds have been fabricated to recreate complex extracellular matrix (ECM) environment support desired cell behaviors; however, it is often challenging design with requisite cell-anchorage sites, mechanical stability, and tailorable physicochemical properties necessary for many applications. To address this improve on hyaluronic acid (HA) hydrogels, we combined photocrosslinkable norbornene-modified HA (NorHA) human platelet lysate (PL). These PL-NorHA hybrid hydrogels supported adhesion cells when compared NorHA without PL, exhibited based concentration individual components, released proteins over time. Using microfluidic techniques on-chip mixing PL subsequent photocrosslinking, spherical microgels a hierarchical fibrillar network were that sustained delivery proteins. Microgels could be jammed into granular shear-thinning self-healing properties, enabling ejection from syringes fabrication stable 3D constructs printing. Again, inclusion enhanced cellular interactions microgel structures. Overall, combination biomolecules fibrin self-assembly arising enriched milieu PL-derived improved bioactivity HA-based formation dynamic systems modular design. The can engineered meet demands functional tissue using versatile processing techniques, such as